soap的結果分析：
1) id of read
2) full sequence of read. the read will be converted to the complementary if mapped on the reverse chain of the reference;
3) quality of the sequence corresponding to sequence.
4) number of hits. #全部比對上的次數
5) a/b, flag only meaningful for pair-end alignment.
6) length of read.
7) alignment on the direct(+) or reverse(-) chain of the reference.
8) location of first bp on the reference, counted from 1.
9) types of hits.
0: exact match.
n: the number of mismatch
10) Reference allele->Offset--Query allele--Quality
11) n—M, Offset--Reference allele--Quality
example:
SRR068443.6657 CTACAAAGGACATGAACTCATGATTTTTTATGGCTGCATAGTATTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCTATCATTGTTGGACATTTG de`ddcecfbcdbffdcbcfee``fffe`ffff^fadaffdfafffeefffffcffeffffffceffee`ffefcfefffdefffffffffcffeffffff 800 a 101 - chr1 74158 1 C->21G37 101M 21C79
SRR068443.6657 GTCCCCAGAGTGTGATATTCCCCTTCCTGTGTCCATGTGATCTCATTGTTCAATTCCCACCTATGAGTGAGAATATGCGGTGTTTGGTTTTTTGTTCTTGC ffffffeecf^dddc^eeeefffcfffdf^d`dddeeeecceffcffffff^ffefffdfffdffafcfbdcbc^aY^c_Wcaaabbbb^eceabb\d_^a 800 b 101 + chr1 74014 1 C->52A38 101M 52C48
74001 CCACCCCACAACAGTCCCCAGAGTGTGATATTCCCCTTCCTGTGTCCATG 74050
74051 TGATCTCATTGTTCACTTCCCACCTATGAGTGAGAATATGCGGTGTTTGG 74100
74101 TTTTTTGTTCTTGCGATAGTTTACTGAGAATGATGATTTCCAGTTTCATC 74150
74151 CATGTCCCTACAAAGGACATGAACTCATCATTTTTTATGGCTGCATAGTA 74200
74201 TTCCATGGTGTATATGTGCCACATTTTCTTAATCCAGTCTATCATTGTTG 74250
74251 GACATTTGGGTTGGTTCCAAGTCTTTGCTATTGTGAATAATGCCGCAATA 74300
74301 AACATACGTGTGCATGTGTCTTTATAGCAGCATGATTTATAGTCCTTTGG 74350
74351 GTATATACCCAGTAATGGGATGGCTGGGTCAAATGGTATTTCCAGTTCGA 74400
74401 GATCCCTGAGGAATCGCCACACTGACTTCCACAATGGTTGAACTAGTTTA 74450
74451 CAGTCCCACCAACAGTGTAAAAGTGTTCCTATTTCTCCACATCCTCTCCA 74500
怎麼判斷插入序列的長度是多少？