Moving Towards Third‐Generation Sequencing Technologies
邁向第三代測序技術
Within just the past few years, next‐generation sequencing (NGS) platforms have reduced the cost of DNA sequencing by several orders of magnitude. With these lower costs, DNA sequencing is increasingly powerful as a single‐data format onto which a broad range of biological processes can be projected for high‐throughput molecular profiling. Although significant challenges remain, translating these powerful technologies into useful laboratory tests has great potential. This chapter provides a comparative analysis of currently available NGS techniques, including those of Illumina, 454/Roche, Applied Biosystems, and Helicos BioSciences, and addresses emerging techniques, such as Pacific Biosciences single‐molecule sequencing and nanopore sequencing. Furthermore, very recent electronic sequencing from Ion Torrent is elaborated. The chapter discusses advantages and disadvantages of individual platforms, and those common to all techniques, including template preparation and genome enrichment.
就在過去的幾年裏，下一代測序(NGS)平臺已經將DNA測序的成本降低了幾個數量級。有了這些較低的成本，DNA測序作爲一種單一的數據格式變得越來越強大，在這種數據格式上，可以預測出大範圍的生物過程，從而進行高通量的分子分析。儘管仍存在重大挑戰，但將這些強大的技術轉化爲有用的實驗室測試具有巨大的潛力。本章對現有的NGS技術，包括Illumina、454/Roche、Applied Biosystems和Helicos BioSciences進行了比較分析，並介紹了新興的技術，如Pacific BioSciences的單分子測序和nanopore測序。此外，還闡述了近年來離子激流的電子測序方法。本章討論了單個平臺的優點和缺點，以及所有技術的共同之處，包括模板製備和基因組富集。
參考文獻
Ting Gao, Zhichao Xu, Xiaojun Song, Kai Huang, Ying Li, Jianhe Wei, Xunzhi Zhu, Hongwei Ren and Chao Sun, Hybrid Sequencing of Full-Length cDNA Transcripts of the Medicinal Plant Scutellaria baicalensis, International Journal of Molecular Sciences, 10.3390/ijms20184426, 20, 18, (4426), (2019).
