跟着Nature Communications學作圖：R語言ggplot2做堆積柱形圖展示羣體基因組學的結果

論文

Genomic insights into local adaptation and future climate-induced vulnerability of a keystone forest tree in East Asia

https://www.nature.com/articles/s41467-022-34206-8#Sec23

完整的數據分析代碼 涉及到羣體基因組學

作圖數據 ``

https://github.com/jingwanglab/Populus_genomic_prediction_climate_vulnerability

作者的github主頁還有很多其他內容 https://github.com/jingwanglab

今天的圖推文重複一下論文中的figure2a

論文中提供的代碼是

https://github.com/jingwanglab/Populus_genomic_prediction_climate_vulnerability/blob/main/3-Population_genetics/1structure.sh

完整代碼

Q2=read.table("pk230_ldpruned.2.Q.txt",header=F)
dim(Q2)
Q3=read.table("pk230_ldpruned.3.Q.txt",header=F)
dim(Q3)

myorder <- c("ZHY-03-1","ZHY-03-10",
             "ZHY-03-12","ZHY-03-17",
             "ZHY-03-2","ZHY-03-3",
             "ZHY-03-4","ZHY-03-6",
             "ZHY-03-7","ZHY-03-9",
             "ZHY-09-1","ZHY-09-11","ZHY-09-15",
             "ZHY-09-16","ZHY-09-17","ZHY-09-18",
             "ZHY-09-2","ZHY-09-6","ZHY-09-8","ZHY-10-1",
             "ZHY-10-11","ZHY-10-13","ZHY-10-14",
             "ZHY-10-15","ZHY-10-16","ZHY-10-3",
             "ZHY-10-4","ZHY-10-6","ZHY-10-9",
             "LiuJQ-MZL-2013-249-1","LiuJQ-MZL-2013-249-10",
             "LiuJQ-MZL-2013-249-3","LiuJQ-MZL-2013-249-4",
             "LiuJQ-MZL-2013-249-5","LiuJQ-MZL-2013-249-6",
             "LiuJQ-MZL-2013-249-7","LiuJQ-MZL-2013-249-8",
             "LiuJQ-MZL-2013-249-9","LiuJQ-MZL-2013-262-1",
             "LiuJQ-MZL-2013-262-10","LiuJQ-MZL-2013-262-11",
             "LiuJQ-MZL-2013-262-3","LiuJQ-MZL-2013-262-5",
             "LiuJQ-MZL-2013-262-6","LiuJQ-MZL-2013-262-7",
             "LiuJQ-MZL-2013-262-8","LiuJQ-MZL-2013-262-9",
             "LiuJQ-MZL-2013-283-1","LiuJQ-MZL-2013-283-10",
             "LiuJQ-MZL-2013-283-12","LiuJQ-MZL-2013-283-15","LiuJQ-MZL-2013-283-3","LiuJQ-MZL-2013-283-4","LiuJQ-MZL-2013-283-5","LiuJQ-MZL-2013-283-6","LiuJQ-MZL-2013-283-8","LiuJQ-MZL-2013-283-9","LiuJQ-MZL-2013-297-1","LiuJQ-MZL-2013-297-10","LiuJQ-MZL-2013-297-2","LiuJQ-MZL-2013-297-3","LiuJQ-MZL-2013-297-4","LiuJQ-MZL-2013-297-5","LiuJQ-MZL-2013-297-6","LiuJQ-MZL-2013-297-7","LiuJQ-MZL-2013-297-8","LiuJQ-MZL-2013-297-9","ZHY-14-1","ZHY-14-12","ZHY-14-13","ZHY-14-2","ZHY-14-3","ZHY-14-4","ZHY-14-5","ZHY-14-6","ZHY-14-7","ZHY-14-9","ZHY-16-1","ZHY-16-12","ZHY-16-13","ZHY-16-14","ZHY-16-15","ZHY-16-2","ZHY-16-3","ZHY-16-4","ZHY-16-6","ZHY-16-8","ZHY-17-1","ZHY-17-12","ZHY-17-13","ZHY-17-14","ZHY-17-15","ZHY-17-5","ZHY-17-6","ZHY-17-8","ZHY-17-9","ZHY-18-10","ZHY-18-13","ZHY-18-2","ZHY-18-3","ZHY-18-4","ZHY-18-5","ZHY-18-7","ZHY-18-8","ZHY-18-9","ZHY-19-10","ZHY-19-11","ZHY-19-12","ZHY-19-13","ZHY-19-14","ZHY-19-15","ZHY-19-5","ZHY-19-6","ZHY-19-8","ZHY-19-9","ZHY-21-1","ZHY-21-11","ZHY-21-12","ZHY-21-14","ZHY-21-2","ZHY-21-3","ZHY-21-4","ZHY-21-5","ZHY-21-7","ZHY-21-8","ZHY-22-1","ZHY-22-10","ZHY-22-11","ZHY-22-12","ZHY-22-3","ZHY-22-6","ZHY-22-7","ZHY-22-8","ZHY-22-9","LiuJQ-MZL-2013-323-0","LiuJQ-MZL-2013-323-10","LiuJQ-MZL-2013-323-11","LiuJQ-MZL-2013-323-12","LiuJQ-MZL-2013-323-13","LiuJQ-MZL-2013-323-4","LiuJQ-MZL-2013-323-5","LiuJQ-MZL-2013-323-6","LiuJQ-MZL-2013-323-7","LiuJQ-MZL-2013-323-9","ZHY-25-10","ZHY-25-11","ZHY-25-12","ZHY-25-13","ZHY-25-14","ZHY-25-3","ZHY-25-4","ZHY-25-7","ZHY-25-8","ZHY-25-9","ZHY-26-1","ZHY-26-10","ZHY-26-11","ZHY-26-12","ZHY-26-13","ZHY-26-15","ZHY-26-2","ZHY-26-3","ZHY-26-4","ZHY-26-8","ZHY-31-1","ZHY-31-10","ZHY-31-11","ZHY-31-12","ZHY-31-2","ZHY-31-3","ZHY-31-4","ZHY-31-7","ZHY-31-8","ZHY-33-1","ZHY-33-10","ZHY-33-11","ZHY-33-12","ZHY-33-3","ZHY-33-6","ZHY-33-7","ZHY-33-8","ZHY-33-9","ZHY-34-1","ZHY-34-11","ZHY-34-12","ZHY-34-13","ZHY-34-14","ZHY-34-2","ZHY-34-4","ZHY-34-5","ZHY-34-7","ZHY-34-9","ZHY-35-1","ZHY-35-10","ZHY-35-2","ZHY-35-3","ZHY-35-4","ZHY-35-5","ZHY-35-6","ZHY-35-7","ZHY-35-8","ZHY-35-9","ZHY-37-10","ZHY-37-11","ZHY-37-12","ZHY-37-15","ZHY-37-2","ZHY-37-3","ZHY-37-4","ZHY-37-6","ZHY-37-8","ZHY-37-9","ZHY-41-1","ZHY-41-10","ZHY-41-11","ZHY-41-12","ZHY-41-13","ZHY-41-2","ZHY-41-4","ZHY-41-6","ZHY-41-7","ZHY-41-9","ZHY-44-1","ZHY-44-10","ZHY-44-2","ZHY-44-3","ZHY-44-4","ZHY-44-5","ZHY-44-6","ZHY-44-9")
length(myorder)

library(tidyverse)
p1<-Q2 %>% 
  mutate(V1=factor(V1,
                   levels = myorder)) %>% 
  pivot_longer(-V1) %>% 
  mutate(name=factor(name,levels = c("V3","V2"))) %>% 
  ggplot(aes(x=V1,y=value,fill=name))+
  geom_bar(stat='identity',width=1,show.legend = FALSE)+
  scale_fill_manual(values = c("V3"="#e9e9e9",
                               "V2"="#e04d72"))+
  theme_bw()+
  theme(panel.grid = element_blank(),
        axis.text.x = element_blank(),
        axis.ticks.x = element_blank())+
  scale_y_continuous(minor_breaks=seq(0,1,0.1),
                     expand = c(0,0),
                     breaks=seq(0,1,0.25))+
  scale_x_discrete(breaks=NULL)+
  labs(x=NULL,y="k=2")


p2<-Q3 %>% 
  mutate(V1=factor(V1,
                   levels = myorder)) %>% 
  pivot_longer(-V1) %>% 
  #mutate(name=factor(name,levels = c("V3","V2"))) %>% 
  ggplot(aes(x=V1,y=value,fill=name))+
  geom_bar(stat='identity',width=1,show.legend = FALSE)+
  scale_fill_manual(values = c("V2"="#e9e9e9",
                               "V3"="#3280c3",
                               "V4"="#e04d72"))+
  theme_bw()+
  theme(panel.grid = element_blank(),
        axis.text.x = element_blank(),
        axis.ticks.x = element_blank())+
  scale_y_continuous(minor_breaks=seq(0,1,0.1),
                     expand = c(0,0),
                     breaks=seq(0,1,0.25))+
  scale_x_discrete(breaks=NULL)+
  labs(x=NULL,y="k=3")

p3<-Q2 %>% 
  mutate(V1=factor(V1,
                   levels = myorder)) %>%
  ggplot()+
  geom_ribbon(aes(x=V1,ymin=0.1,ymax=1),fill="#e04d72")+
  #geom_ribbon(aes(x=164:230,ymin=0.1,ymax=1),fill="#3280c3")+
  theme_bw()+
  theme(panel.grid = element_blank(),
        axis.text = element_blank(),
        axis.ticks = element_blank(),
        panel.border = element_blank(),
        axis.title = element_blank())+
  scale_y_continuous(minor_breaks=seq(0,1,0.1),
                     expand = c(0,0),
                     breaks=seq(0,1,0.25))+
  #scale_x_continuous(breaks=NULL)+
  annotate(geom="text",x=80,y=0,label="South",vjust=-0.5)+
  annotate(geom="text",x=190,y=0,label="North",vjust=-0.5)+
  annotate(geom = "ribbon",x=1:165,ymin=0.5,ymax=1,fill="#e04d72")+
  annotate(geom = "ribbon",x=166:230,ymin=0.5,ymax=1,fill="#3280c3")

library(patchwork)

p1/p2/p3+
  plot_layout(heights = c(4,4,1))

最終結果

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