Over the past decades, it has become almost routine to determine the identity and order of nucleotide bases in any piece of nucleic acid. DNA sequencing has evolved from analysing one nucleotide at a time, by using costly and time-consuming assays, to massively parallel ‘next- and third-generation sequencing’ approaches. Over the past 15 years, substantial reductions in the cost of DNA sequencing have been achieved. This tremendous progress has resulted in a wealth of opportunities when embarking on a sequencing project. In this chapter, therefore, we discuss the most frequently applied past and current technological possibilities in DNA sequencing and identify their strengths and weaknesses.
在過去的幾十年裏，確定核酸中核苷酸鹼基的身份和順序已經成爲一種慣例。
DNA測序已經從一次分析一個核苷酸，通過使用昂貴和耗時的分析，發展到大規模並行的“第二代和第三代測序”方法。
在過去的15年裏，DNA測序的成本大幅降低。這一巨大的進步在啓動一個排序項目時帶來了大量的機會。
因此，在這一章中，我們將討論DNA測序中最常用的過去和現在的技術可能性，並找出它們的優缺點。